ABSTRACT
Deedan is a very effective compound formulation of Unani System of medicine used for the treatment of worm infestation. The objective of this study was to investigate the Acute and Sub-acute toxicity of Deedan in Albino rats of both the sexes. In the acute toxicity study, Deedan was administered orally at the limit dose of 2000mg/kg b.w. to both male and female rats, and the animals were then observed individually 30 minutes, 4 hour postdosing, and at least twice daily for next 14 days. In the Sub-acute toxicity study a limit dose of 1000 mg/kg body weight was administered orally in a single bolus everyday for 28 days. The rats were observed daily during the period of study, and sacrificed on the 29th day. Observation parameters of the animals included a comparative evaluation of general appearance/behaviour, morbidity/mortality, body weights, food/water consumption, haematology, biochemistry and histopathology of major organs of treated and control groups. There was no mortality, morbidity, or cage-side/laboratory findings of any adverse health effect in the treated animals in comparison to their respective controls in both toxicity studies. Deedan was thus found to be free of any toxic effects under the conditions of these studies.
ABSTRACT
Unani system of medicine is gaining popularity day by day owing to its efficiency, low cost, availability and to the safety of its formulations and drugs/medicines. The current study was aimed to determine the safety of a polyherbal Unani formulation named Deedan. A suspension of powdered Deedan at the dose of 300mg/kg of b. wt was orally administered to the male and female group of rats (n=5) for 90 days daily and then sacrificed. The biochemical and hematological parameters of treated rats were compared with respective control group of rats. Various vital organs/tissues were collected, examined, weighed and tissue samples were taken for histopathological studies of both the treated and control groups. Physiological parameters like water and feed consumption were also observed in all the groups. The treated rats (both males and females) were found to gain bodyweight normally. There was no effect of the drug on biochemical and hematological parameters as the changes were statistically insignificant. The histopathological studies revealed the normal appearance of tissues. The feed and water consumption in treated group of rats was found to be unaffected by the drug administration. The drug deedan was found to be safe at the dose of 300 mg/kg of body weight when administered orally to the rats for 90 days.